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<div class="ng4emdn4q" id="ny_biaoti"><span>您現(xiàn)在的位置：<a href="/">首頁</a> > <a href="/news.html">新聞中心</a> > 人白介素ELISA試劑盒的主要組成部分功能特點(diǎn)分享</span></div> <div id="ng4emdn4q" class="about"> <div class="ng4emdn4q" id="newscont"> <h1 class="newsname">人白介素ELISA試劑盒的主要組成部分功能特點(diǎn)分享</h1> <li id="ng4emdn4q" class="time">更新時(shí)間:2025-03-13 瀏覽次數(shù)：19 </li> <ul class="dcont"> <div>　　人白介素ELISA試劑盒是一種專門用于定量檢測人體樣本中白介素（Interleukin，簡稱IL）含量的工具。其基于雙抗體夾心法原理，通過酶聯(lián)免疫吸附試驗(yàn)（ELISA）實(shí)現(xiàn)檢測。該試劑盒可檢測多種類型的白介素，如IL-2、IL-3、IL-6等，適用于血清、血漿、尿液、細(xì)胞培養(yǎng)上清液及組織標(biāo)本等多種樣本類型。</div><div> </div><div>　　以下是<a href="http://m.erdemann.cn/Products-37025453.html" target="_blank">人白介素ELISA試劑盒</a>的主要組成部分及其功能特點(diǎn)，希望對您有所幫助。</div><div> </div><p align="center"><img src="https://img58.chem17.com/9/20250313/638774610738521674985.jpg" width="500" height="500" align="middle" alt="" /></p><p> </p><div>　　1、包被板</div><div> </div><div>　　功能：包被板是ELISA實(shí)驗(yàn)的基礎(chǔ)平臺，通常為96孔格式，每個(gè)孔都預(yù)先涂覆有特異性捕獲抗體，這些抗體能夠識別并結(jié)合目標(biāo)人白介素。</div><div> </div><div>　　特點(diǎn)：高質(zhì)量的包被板確保了抗體牢固地附著在孔壁上，并且具有低背景噪音，有助于提高檢測靈敏度和重復(fù)性。</div><div> </div><div>　　2、標(biāo)準(zhǔn)品</div><div> </div><div>　　功能：標(biāo)準(zhǔn)品包含已知濃度的人白介素，用于建立標(biāo)準(zhǔn)曲線，從而根據(jù)樣本OD值推算出未知樣本中目標(biāo)分子的確切濃度。</div><div> </div><div>　　特點(diǎn)：精確配制的標(biāo)準(zhǔn)品提供了可靠的參照點(diǎn)，幫助用戶校準(zhǔn)測試結(jié)果。不同濃度的標(biāo)準(zhǔn)品覆蓋了廣泛的動(dòng)態(tài)范圍，適合各種樣本類型的檢測需求。</div><div> </div><div>　　3、酶標(biāo)二抗</div><div> </div><div>　　功能：酶標(biāo)二抗是在免疫反應(yīng)之后加入的，它能特異性地與一抗結(jié)合，并攜帶一個(gè)酶標(biāo)記物，如辣根過氧化物酶（HRP）或堿性磷酸酶（AP），以便后續(xù)顯色反應(yīng)。</div><div> </div><div>　　特點(diǎn)：高效的酶標(biāo)二抗增強(qiáng)了信號放大效果，使得即使微量的目標(biāo)蛋白也能被有效檢測出來。選擇合適的酶標(biāo)記物對提高檢測靈敏度至關(guān)重要。</div><div> </div><div>　　4、顯色底物</div><div> </div><div>　　功能：當(dāng)酶標(biāo)二抗結(jié)合到復(fù)合體后，加入顯色底物可以引發(fā)顏色變化，這種變化可通過光吸收測量來量化。</div><div> </div><div>　　特點(diǎn)：理想的顯色底物應(yīng)具備高靈敏度、快速響應(yīng)以及穩(wěn)定性好等特點(diǎn)。常見的底物如TMB（四甲基聯(lián)苯胺）適用于HRP標(biāo)記系統(tǒng)，而PNPP（對硝基苯磷酸鹽）則常用于AP標(biāo)記系統(tǒng)。</div><div> </div><div>　　5、終止液</div><div> </div><div>　　功能：終止液用于停止顯色反應(yīng)，防止過度顯色影響讀數(shù)準(zhǔn)確性。</div><div> </div><div>　　特點(diǎn)：酸性溶液是常用的終止劑之一，它可以迅速降低pH值以中斷酶促反應(yīng)，同時(shí)使溶液顏色固定下來便于光度計(jì)讀取吸光度值。</div><div> </div><div>　　6、洗滌緩沖液</div><div> </div><div>　　功能：洗滌步驟旨在去除未結(jié)合的物質(zhì)，減少非特異性結(jié)合引起的誤差。</div><div> </div><div>　　特點(diǎn)：良好的洗滌緩沖液不僅能有效地清洗掉多余的試劑，還能保持結(jié)合物的完整性，避免不必要的損失。</div><div> </div><div>　　通過上述介紹可以看出，人白介素ELISA試劑盒的組件都在整個(gè)實(shí)驗(yàn)流程中扮演著重要的角色。正確理解和應(yīng)用這些組件的功能特性，對于獲得可靠的數(shù)據(jù)至關(guān)重要。此外，嚴(yán)格遵循操作指南進(jìn)行實(shí)驗(yàn)準(zhǔn)備、執(zhí)行及數(shù)據(jù)分析也是保證結(jié)果準(zhǔn)確性的關(guān)鍵因素。</div> </ul> <div class="ng4emdn4q" id="news_sx"> <li id="ng4emdn4q" class="sx_left">上一篇：沒有了 <br />下一篇：<a href="/News-1605179.html">簡述選購大鼠低氧誘導(dǎo)因子1αelisa試劑盒時(shí)所需要考慮的關(guān)鍵因素</a></li> <li id="ng4emdn4q" 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